Identification and counting of granulocytes by peroxidase reaction.

By TAPIO RYTOMAA P HLOXINE in propylene glycol provides a good and simple method of staining for the counting of eosinophilic granulocytes in the counting chamber. No comparable method has been available for the counting of neutrophils. The identification of cell types in certain excreta of the organism, especially in the intestinal contents, is a cumbersome procedure.”2 It has, however, proved necessary for quantitative studies of granulocyte kinetics.2’3 The present experimental investigation introduces a staining method based on the utilization of myeloperoxidase, an enzyme specific for the myeloid cell series.4-6 This method makes it possible to differentiate between granulocytes and other cells both in the counting chamber and in smears.